blood culture collection order of draw

Blood Collection Container Information. This is known as the Order of Draw.

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RNs carry a medical directive to order cultures when indicated.

. Coagulation Tubes Blue-Top Tubes Serum Tubes without Clot Activator or Gel Red-Top tubes Serum Tubes with Clot Activator or Gel Gold or Tiger Top Tubes Heparin Tubes Green-Top Tubes. Blood Collection ORDER OF DRAW MIXING CHART SEPARATION Clot Spin Pour into Transport Vial Label with specimen type and patient ID For other blood serum or plasma separation instructions see the Specimen Collection and Handling section of this directory. Blood culture tube or bottle.

Confirm that MDs order for blood culture specifies a line draw. Citrate tube blue Fill to mark as the ratio of blood to anticoagulant is critical. Blood Collection order of draw for tubes and bottles Tube Type Tests Instructions Blood Culture Bottles Blood Culture Ensure aseptic technique.

An order must be obtained Draw cultures before the initiation of antibiotic therapy if possible. Blood Draw Order Tube Color NameAdditive of Inversions. Draw stations staffed by Pathology.

Blood Culture Bottles 2. BD Vacutainer Tube Type Number of Inversions 2 3 4 5 6 7 8 9 1. Skin Puncture Order of Collection PA03-010.

Blood Draw Order Tube ColorDescription ShowHide All. The correct order of draw follows. Blood Culture Tubes or Vials.

The above order of draw is to be followed. Collect specimens for each printed container label. Phlebotomy order of draw is the same for specimens collected by syringe tube holder or into tubes preevacuated at the time of collection.

Light Blue Sodium Citrate. Collect blood culture from second site if. Label every specimen container with the correct container label.

Light Green Lithium Heparin. 1 Blood cultures yellow SPS sterile 2 Light blue buffered sodium citrate tube 3. Blood should not be drawn from a VAD unless line related sepsis is suspected.

Factors Affecting Blood Test Results. Blood Cultures Sterile Tubes. When collecting diagnostic blood specimens by venipuncture into evacuated tubes the order of draw is b ased on Clinical and Laboratory Standards Institutes CLSI standard H3-A6 from November 2007 as follows.

Citrate Tube Light Blue SST TM Gel Separator Tube Red or Gold or Serum Tube Plastic or Glass - Red PST TM Gel Separator Tube Green or Heparin Tube Sodium or Lithium - Green EDTA Tube Lavender Fluoride Tube Grey. SUPPLIES Requisition and labels Sterile packaged gloves 2 10mL sterile syringes 2 Sterile BD transfer devices Safety-Lok blood collection set 21 or 23 gauge CloraPrep One Step Applicator either 15 mL or 3 mL 3-4 sterile 70 alcohol swabs 1 anaerobic culture bottle. And Order of Draw see end of page __Cap.

Color Varies Blood Cultures. Correct order of draw. ORDER OF DRAW FOR BLOOD COLLECTION Collect blood into containerstubes in the following order.

The label must be affixed directly to the container. Draw refer to Prompt Document. Sodium citrate tube eg blue closure Serum tubes including those with clot activator and gels eg red red-speckled gold closures.

Order of DrawCollection Collect blood cultures first starting with the aerobic vial followed by the anaerobic lytic vial from the first site. The recommended order of draw is as follows. Shaking the tube vigorously may cause hemolysis of the blood separating of red blood cells.

ESR tube black 5. Any order for blood cultures should consist of a MINIMUM of 2 complete sets of cultures. Laboratory Process for the Collection of Blood.

Specimens must be submitted either with a requisition or by computer order. Ordering Blood Cultures in CCTC. Drawing Blood Cultures from vascular access device VAD.

Fluoride Oxalate Glucose Alcohol Lactate 368920 Draw Volume 2ml Mix 3-4 Times Mix 8-10 Times Mix 5-6 Times Mix 6 Times Mix 8-10. Yellow SPS No paper label TB Cultures Royal Blue BD brand No additive Trace Elements Serum TRELS Aluminum Chromium Copper Nickel Selenium Zinc If a blood culture has been collected a new venipuncture is required. Sodium Fluoride Potassium Oxalate.

Dark Green Sodium Heparin. Serum gel gold serum no gel tube red 4. Evidence supporting the need for a specific order in which blood collection tubes should be filled was first published over 30 years ago yet the concept remains elusive to many healthcare.

Order of Draw When Using Evacuated Tubes. Remove tourniquet for skin preparation. Blood Culture Bottles Used for bacterial and.

All tubes must be gently inverted 8 10 times to provide thorough mixing of additives. SST Serum Separator Tube 4 5. Order of Draw for Vacutainer Collection Tubes 1.

Order Of Draw and Order of Transfer PA3-005. When collecting blood via a capillary collection method heelfinger prick or when using a syringe and siphoning the bloods directly into the tubes the Order of Draw in order is. To avoid cross-contamination blood must be drawn and collected in tubes in a specific order.

Each set of cultures should be drawn from a different draw eg 2 different venipuncture sites or a venipuncture plus line sites. Red Clot Activator. Intraosseous IO Blood Specimen Submission to the Laboratory.

Trace Elements Trace Elements 368381 Draw Volume 6ml Cat. Color Description_____Instructions___ Blood culture bottles Contains anticoagulants and preservatives to culture bacteria. Laboratory Policy on Performing Blood Collections for a Peace Officer.

CUsersjaj04DesktopmemorialBlood Collection Container Information and Order of Drawdocx. Blood Gas Syringe EDTA tubes. Draw Volume 4ml Cat.

Lithium Heparin tube green 6. Blood cultures drawn from lines are more likely to be contaminated therefore adequate precautions should be taken to avoid contamination. Do not draw blood cultures from lines unless there is absolutely no alternative site.

Draw all other blood work ie. Blood Culture Bottles Blue Purple Pink with various additives Blood Cultures Remember to draw the aerobic vial first. Chemistry Hematology etc from this site after blood culture has been collected.

The Order of Draw. EDTA Blood Transfusion Group Screen Crossmatch Antenatal blood group DATDCT KleihauerFetal Cell Count 367941 Draw Volume 6ml Cat. See Step 3b if no alternative for Intravascular Catheter draw.

Specimens must be collected in the proper containers in the correct order-of-draw above. Order of blood draw. Do I Have To Follow It.

Collect Aerobic then Anaerobic APTT Coagulation profile D-dimer Factor assays heparin assay INR Lupus anticoagulant ratio of blood to Fill to mark as the. When collecting blood using a syringe and a transfer device is used.

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